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Goals:
In biolab.
Methods:
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Taking samples at three locations: spring, halfway down the stream and confluence.
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Total count of all bacteria.
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Coliform bacteria count.
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Sampling and the determination of phytocenosis and zoocenosis of stream´s ecosystem.
Sampling results are shown in Tables 1, 2, 3 and 4.
Table 1. Results of microscopic analysis of fresh water and bed samples
| Findings |
Number of smears |
Percentage (%) |
| Determined bacteria |
94 |
52,98% |
| Undetermined bacteria |
86 |
47,02% |
| Total |
180 |
100% |
Table 2. Results of microscopic analysis of
Gram - stained smears
| Findings |
Number of smears |
Perentage (%) |
| Gram - negative bacteria |
78 |
82,98% |
| Gram - positive bacteria |
16 |
17,02% |
| Total |
94 |
100% |
Table 3. Distribution of bacteria grown in Endo -
Agar
|
Agent |
Number |
Percentage (%) |
| Escherihia coli |
90 |
77,59% |
| Enterobacter |
8 |
6,9% |
| Proteus |
6 |
5,17% |
| Enterococus (streptococus faecalis) |
9 |
7,76% |
| Clostridium perfringeus |
3 |
2,58% |
| Total |
116 |
100% |
Another method – that of cultivating Water (all 180 samples), has provided as water the insight into individual bacteria kinds. 116 samples were positive (108 with many bacteria), and 64 samples were negative. Out of 116 positive ones, the most frequent grown colonies were those of Escherichia coli, followed by Enterobacter, Proteus, Enterococcus and Clostridium Perfringeus.
180 samples containing the Big Creek water were subjected to microscopic analysis (Table 1). Gram – stained smears showed bacteria in 94 smears, while no bacteria developed in 86 smears. Out of 94 smears, 78 contained G- (Gram – negative) and 16 G+ (Gram – positive) bacteria – Table 2.
Table 4. Sampling results of individual biocenosis at the three locations of the Big Creek
| Biocenosis |
P1 |
P2 |
P3 |
| Flora |
Euglena viridis (euglena) |
Euglena viridis |
|
Volvox
(green algae) |
Blue-green algae |
Blue-green algae |
Ulothrix
(green algae) |
Green algae |
|
| Cladophora (green algae) |
Diatomae |
|
| Lichenes (lichenes) |
|
|
Diatomae
(single - cell algae) |
|
|
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|
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| Fauna |
Paramecium |
Paramecium |
|
| Nematod worms (larvar) |
Mosquito larvae |
|
| Hygrabia tarda (larvae) |
Trichoptera |
Trichoptera |
Insect
(larvae) |
|
|
| Trichoptera |
|
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Conclusions:
The different sampling methods and obtained results show the adverse urban, rural, and industrial effects on the biodiversity of living
world.
Another pichture of biolab.
The importance of preserving biodiversity of species as well as of biodiversity of all living organisms in the environment is comprised in the influence of one species onto the other and consequently onto man as well. Man, polluting water, forgets that it flows downstream where he
lives.
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